温州医科大学眼视光学院 眼视光学和视觉科学国家重点实验室,温州 325027
国家自然科学基金(81201181)和浙江省科技计划 (2017C37176)资助项目
State Key Laboratory of Ophthalmology and Optometry, School of Ophthalmology and Optometry, Wenzhou Medical University,Wenzhou 325027, Zhejiang, China
This work was supported by grants from The National Natural Science Foundation of China (81201181), Science and Technology Project of Zhejiang Province (2017C37176)
近年来,基因组编辑技术得到了飞速发展,该技术正在基础生物学研究、医学、生物技术等多个领域引起一场新的变革.Cpf1,作为CRISPR系统的新成员,极大地扩展了基因编辑靶位点的选择范围,同时其介导的多基因编辑具有明显的优势.另外,较短的crRNA序列也使Cpf1更容易产业化.本文将从Cpf1的结构和编辑特点、应用进展、目前面临的问题及展望等方面进行介绍和总结.
In recent years, genome editing technology has been well developed, which have major implications for basic biology research, medicine, and biotechnology. As a new member of CRISPR system, Cpf1 greatly expands the choice of target sites for genome editing and has significant advantages in multiple gene editing. In addition, its shorter RNA sequences (crRNAs) make it closer for the application via industrial direct synthesis. Here we summarized the structure, gene editing characteristics, application progress, current limitations and future prospects of Cpf1 system.
周晨晨,刘写写,谢海华,谷峰. CRISPR家族新成员:CRISPR-Cpf1[J].生物化学与生物物理进展,2018,45(6):585-592
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