中国科学院生物物理研究所蛋白质科学研究平台;中国科学院生物物理研究所蛋白质科学研究平台,中国科学院生物物理研究所蛋白质科学研究平台;中国科学院生物物理研究所蛋白质科学研究平台,华中科技大学生命与技术学院,中国科学院生物物理研究所科教融合生物大分子卓越创新中心,中国科学院大学,中国科学院生物物理研究所蛋白质科学研究平台;中国科学院生物物理研究所蛋白质科学研究平台
国家自然科学基金重大仪器研制项目(31127901)资助
Institute of Biophysics, Chinese Academy of Sciences,Institute of Biophysics, Chinese Academy of Sciences,College of Life Science and Technology, Huazhong University of Science and Technology,National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences,University of Chinese Academy of Sciences,Institute of Biophysics, Chinese Academy of Sciences
This work was supported by a grant from The National Natural Science Foundation of China (31127901)
冷冻超分辨光电融合成像技术近年来发展迅速,该技术结合了荧光显微镜特异性标记与冷冻电镜超高分辨率的优势,成为细胞原位结构研究的新手段,有望发展成为下一代成像技术.本文从发展背景、应用领域等几个方面,介绍了冷冻超分辨光电融合成像技术的概况及未来发展前景.
Cryogenic super-resolution correlative light and electron microscopy (csCLEM)has been rapidly developed in recent years. This imaging methodology combines the specific labeling of fluorescence microscopy and the ultra-high resolution of electron microscopy, providing a new tool for resolving cellular structures in situ. It has the potential to become the next generation of imaging method. In this article, csCLEM was reviewd based on its historical background, application fields, development prospects, etc.
李尉兴,谷陆生,徐晓君,薛艳红,纪伟,徐涛.综述: 冷冻超分辨光电融合成像技术 ——新挑战,新机遇[J].生物化学与生物物理进展,2018,45(9):957-960
复制生物化学与生物物理进展 ® 2025 版权所有 ICP:京ICP备05023138号-1 京公网安备 11010502031771号