Objective Learned helplessness (LH) refers to establishment of the helpless state in animals, which have experienced exposure to stressors that are inescapable by means of behavioral responses. It could affect their locomotor activity and other behaviors, such as circadian rhythm, food intake, immune system, etc. LH has been observed in many species including Drosophila melanogaster. Activity of LH flies decreased significantly after inescapable heat shocks. However, it is still unknown if there is impairment in learning ability in LH of Drosophila. Here, we explored if place learning ability of Drosophila could be effected by LH.Methods We established a new experimental paradigm and procedure for place learning and LH experiment in Drosophila.Results We observed robust place learning and LH phenomenon in the new paradigm. We also found that after long-term exposure to inescapable heat pulses their place learning ability was reduced.Conclusion Our findings reveal that LH in Drosophila not only effect their locomotor activity but also place learning behaviour.