胎盘脱细胞基质作为组织修复材料的初步研究
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银丰低温医学科技有限公司,济南 250102

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Tel: 0531-88897323, E-mail: 394995535@qq.comTel: 86-531-88897323, E-mail: 394995535@qq.com

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A Preliminary Study of Placental Acellular Matrix as Tissue Repair Material
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Yinfeng Cryomedicine Technology CO., Ltd., Jinan 250102, China

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    摘要:

    目的 制备胎盘脱细胞基质并评价其生物相容性,探讨其作为组织修复材料的可行性。方法 利用分娩废弃物胎盘组织,进行病毒灭活、脱细胞处理、冷冻干燥得到胎盘脱细胞海绵状基质材料,HE染色观察脱细胞效果及扫描电镜观察材料微观结构。同时,选取健康雄性SD大鼠39只,体质量120~150 g,随机分为实验1组、实验2组及对照组。对构建的基质材料进行大鼠皮下植入实验,实验1组植入基质材料,实验2组植入基质材料及脐带间充质干细胞,对照组为假手术组。于手术后第3、5、7天进行动物血常规检测,分析淋巴细胞、粒细胞等炎性细胞数量;于手术后第1、2、4、8、9周取材料植入处及周围组织样本进行HE染色分析。结果 构建的胎盘脱细胞基质肉眼观呈乳白色海绵状,HE染色未见细胞残留,电镜观察材料内部空隙比较明显,材料交联度较好,总孔隙率为(77.54±2.53)%。皮下植入之后,切口处愈合良好,血常规结果未见明显的炎性细胞增多;术后7 d植入材料切片HE染色即可见血管形成,且脐带间充质干细胞的加入能够加快材料与机体的融合,促进细胞的深入生长及血管化。结论 胎盘脱细胞海绵基质材料具有良好的生物相容性,可作为组织工程材料的理想来源。

    Abstract:

    Objective To prepare and evaluate the biocompatibility of placental acellular matrix and to explore its feasibility as tissue repair material.Methods The waste placental tissue from delivery was handled by virus inactivation, acellular treatment and freeze-drying to obtain the acellular spongy matrix material of placenta. The effect of acellular was observed by HE staining and the microstructure of the materials was observed by scanning electron microscope. At the same time, 39 healthy male SD rats with body mass ranging from 120 g to 150 g were selected, randomly divided into experiment 1 group, experiment 2 group and control group. The subcutaneous implantation of the constructed matrix material was performed in rats, experimental group 1 implanted matrix materials, experimental group 2 implanted matrix materials and umbilical cord mesenchymal stem cells, the control group was the sham operation group. Routine blood tests were performed on the 3rd, 5th and 7th day after surgery to analyze the number of lymphocytes, granulocytes and other inflammatory cells. At 1, 2, 4, 8 and 9 week after the operation, the tissue samples of the implanted material and surrounding tissues were taken for HE staining analysis.Results The constructed acellular matrix of placenta appears milky white spongy in macroscopic view, HE staining indicated no cell residue. Under electron microscope, the internal void of the material was obvious, and the crosslinking degree of the material was good. The total porosity was (77.54±2.53)%. After subcutaneous implantation, the incision healed well, and no obvious inflammatory cell increase was observed in blood routine examination. Seven days after the operation, blood vessel formation can be observed by HE staining in the sections of the implanted materials. Moreover, the addition of umbilical cord mesenchymal stem cells can accelerate the fusion of the materials with the body, promote the in-depth growth of cells and vascularization.Conclusion Placental acellular sponge matrix material has good biocompatibility and can be an ideal source of tissue engineering materials.

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刘琇,臧传宝,林彦超,程龙,付晓萌.胎盘脱细胞基质作为组织修复材料的初步研究[J].生物化学与生物物理进展,2022,49(10):2034-2040

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历史
  • 收稿日期:2021-11-12
  • 最后修改日期:2022-02-16
  • 接受日期:2022-02-16
  • 在线发布日期: 2022-10-21
  • 出版日期: 2022-10-20