1)浙江大学医学院蛋白质平台,杭州 310050;2)浙江大学医学院附属第一医院老年医学科,杭州 310050;3)浙江省增龄与理化损伤性疾病诊治研究重点实验室,杭州 310050
浙江大学实验技术研究项目(SJS202014)和国家自然科学基金(32000707)资助。
1)Protein Facility, Zhejiang University School of Medicine, Hangzhou 310050, China;2)The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310050, China;3)Zhejiang Provincial Key Laboratory for Diagnosis and Treatment of Aging and Physic-chemical Injury Diseases, Hangzhou 310050, China
This work was supported by grants from The Experimental Technique Research Program of Zhejiang University (SJS202014) and The National Natural Science Foundation of China (32000707).
目的 蛋白质纯化是医学与生物学实验教学课程中的一个重要内容,本文通过设计新的蛋白质纯化虚拟仿真教学内容,开发虚拟仿真教学系统,希望学生能够更有效率地掌握蛋白质纯化技术的要点,提升教学质量。方法 利用3D虚拟仿真技术,构建虚拟仿真实验室。结合线下教学经验,确定虚拟仿真教学中需要体现的教学内容、教学重点、核心仪器的特色、评分指标、考核方式、操作体验等。结果 本文基于3D虚拟仿真技术构建了蛋白质纯化虚拟仿真实验室,和传统教学相比,虚拟仿真教学可以提高教学效率,节省教学成本和场地占用;教学设计和虚拟仿真教学系统融合了教学、练习和考核模块,根据以往的教学经验优化了评分体系,保证学生更加有效、严谨地掌握技术细节;涵盖了全新的教学内容——荧光检测联用的分子排阻层析,添加了蛋白质层析的常用案例,保证了内容的新颖与实用;完全再现了现实实验室中仪器设备的搭建模式,实现虚拟学习与实际操作的无缝衔接。结论 蛋白质纯化虚拟仿真教学系统由虚及实,由点及面,把控细节,保证学生动手能力和探索能力的提高,学以致用,并且拥有传统教学模式不可比拟的优势。
Objective Protein chromatography is a fundamental technique in protein sciences. Difficulties were always met in experimental teaching for this technique due to its complexity and tedious details. Therefore, we would like to find new strategies for experimental teaching of such technique.Methods We used the 3D simulation techniques to setup an on-line Protein Chromatography Laboratory. We designed the teaching contents, core equipments, the scoring systems, user interface etc. based on our every-day teaching experiences.Results We took the advantage of the 3D simulation techniques, and designed an on-line Protein Chromatography Laboratory exactly reproduced from the equipment-setups of Protein Facility, Zhejiang University School of Medicine. This virtual simulation teaching platform for protein chromatography contains 3 modules regarding the teaching, practicing and on-line exams, so that the efficiency and quality of teaching could be largely improved. In this VR platform for teaching, a new but commonly used type of protein chromatography equipment was chosen in order to complete such shortages in VR teaching for protein purification. Furthermore, fluorescence-detection size-exclusion chromatography (FSEC) and several case studies were also included in the “advance teaching” section.Conclusion Experimental teaching of protein chromatography using virtual simulation was more advantageous than the “old-fashion” ways of teaching. Students could be involved deeply in learning and practicing of such techniques anywhere beside the chromatography laboratory; and based on our teaching experiences, their learning efficiency and quality were indeed improved.
马骋,宫彩霞.蛋白质纯化虚拟仿真课程的教学设计[J].生物化学与生物物理进展,2022,49(10):2063-2074
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