N-methyl-D-asparate-receptors (NMDAR) are implicated in several neuropathological conditions including epilepsy. As a model of epilepsy, genetically epilepsy-prone rat was chosen to invesitegate the changes in expression of NMDAR1 mRNA after seizure in different brain regions. The results showed that cerebral cortex, hippocampus, subcortex and inferior colliculus had a time-dependent modulation of the NMDAR1 mRNA expression and increased about 111%,113%,165%,202% above control values, respectively in 24h after seizure. This phenomenon demonstrates that NMDAR1 mRNA was regulated by seizure and may contribute to epileptic susceptibility.