采用PCR技术,从佛波酯乙醇刺激的HL-60细胞基因组DNA中扩增获得了1.1 kb的人CD14基因.序列分析表明,有四个碱基发生了突变,其中两处为首次报道.
Human CD14 gene was cloned from genomic DNA of PMA-stimulated HL-60 cells by PCR. Sequencing result showed that it is identical to the reported human CD14 gene except four bases, among which two new mutations were identified.
龙建银,薛沿宁,孙蕾,王会信.人CD14基因的克隆及序列测定[J].生物化学与生物物理进展,1998,25(4):377-378 LONG Jian-yin, XUE Yan-ning, SUN Lei, WANG Hui-xin. Cloning and Sequencing of Human CD14 Gene[J]. Progress in Biochemistry and Biophysics,1998,25(4):377-378
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