采用ESR、自旋捕集及自旋氧探针技术,研究了海藻硫酸多糖(SPS)对豆蔻酰佛波醇乙酯(PMA)刺激的多形核白细胞(PMN)呼吸爆发的影响.结果表明,SPS能显著抑制PMN呼吸爆发,10 g/L和5 g/L SPS几乎完全清除PMN呼吸爆发产生的自由基,1 g/L SPS可清除53.2%;10 g/L SPS对PMN的耗氧量也有较明显的抑制作用.
The influence of sulfated polysaccharide from brown seaweed Laminaria Japonica(SPS) on the respiratory burst of polymorphonuclear neutrophils(PMN) induced by phorbol myristate acetate(PMA) was investigated with the techniques of ESR,spin trap and oxygen spin probe.The results showed that SPS inhibited the respiratory burst of PMN significantly.10 g/L and 5 g/L SPS almost completely scavenged superoxide anion (O-·2) from the respiratory burst while 10 g/L SPS inhibited the oxygen consumption of PMN markablely, and 1 g/L SPS scavenged 53.2% O-·2.
李妍,田晓华,丛建波,滕霞,孙存普,顾景范.海藻多糖抑制白细胞呼吸爆发作用研究[J].生物化学与生物物理进展,1999,26(2):162-164 LI Yan, TIAN Xiao-Hua, CONG Jian-Bo, TENG Xia, SUN Cun-Pu, GU Jing-Fan. Studies on the Inhibitory Effects of Sulfated Polysaccharide from Brown Seaweed on the Respiratory Burst of Polymorophonuclear Neutrophils[J]. Progress in Biochemistry and Biophysics,1999,26(2):162-164
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