Mitogen activated protein kinase (MAPK) is a group of important protein kinases involved in phosphorylation cascade in the mitogen-initiated signal transduction pathways.NGF from Agkistrodon halys has been used to investigate its effects on MAPK and MAPKK of PC12 cells. The results showed that this kind of NGF increased MAPK and MAPKK expression and MAPK activity. The above increases were NGF concentration dependent in the range of 25～100 μg/L. The use of PKC inhibitor H-7 indicated that the increased expressions of MAPK and MAPKK expression were PKC-dependent.