SELF-ASSEMBLING BILAYER LIPID MEMBRANES ON SOLID SUPPORT
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    Abstract:

    Solid supported bilayer lipid membranes (S-BLM) that posses some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a twostep procedure: 1. The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; 2. The new tip of the wire, having become coated with lipid solution, is transferred into 0.1 mol/l KCl. After a few minutes, a stable lipid bilayer forms spontaneously on the tip of wire, as verified by electrical measurements. This paper reports an application of such a supported BLM (S-BLM) in the detection of Pb2+ ions. The S-BLM is liquidcrystalline in structure, which makes it amenable to modification for basic studies, as well as for technological applications such as biobensors and molecular electronic devices.

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H. Ti TIEN, Z. SALAMON. SELF-ASSEMBLING BILAYER LIPID MEMBRANES ON SOLID SUPPORT[J]. Progress in Biochemistry and Biophysics,1990,17(5):359-363

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  • Received:February 08,1990
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