Solid supported bilayer lipid membranes (S-BLM) that posses some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a twostep procedure: 1. The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; 2. The new tip of the wire, having become coated with lipid solution, is transferred into 0.1 mol/l KCl. After a few minutes, a stable lipid bilayer forms spontaneously on the tip of wire, as verified by electrical measurements. This paper reports an application of such a supported BLM (S-BLM) in the detection of Pb2+ ions. The S-BLM is liquidcrystalline in structure, which makes it amenable to modification for basic studies, as well as for technological applications such as biobensors and molecular electronic devices.
H. Ti TIEN, Z. SALAMON. SELF-ASSEMBLING BILAYER LIPID MEMBRANES ON SOLID SUPPORT[J]. Progress in Biochemistry and Biophysics,1990,17(5):359-363
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