Human P. falciparum hybrid peptide antigen gene has been synthesized by the solidphase phosphoramidite method with ABI381A DNA synthesizer. The gene is 216bp in length and divided into 10 fragments to synthesis. All synthetic fragments were annealed and ligated with T4 DNA ligase. The product of synthetic gene was recombinded with phage M13 mp18 vector and transfected to E. coli JM109. The recombinants were screened by spot hybridization with ³²)P-label synthetic fragment. The sequence of synthetic gene was analysed by dideoxynucleotide chain termination method. The recults showed that synthesized human P. falciparum hybrid antigen gene was identical with the designed one.