The nucleotide sequence of the promoter and the signal peptide of E. coli alkaline phosphotase gene from pFOG405 plasmid has been disclosed and compared with the sequence reported by Kikuchi et al. The result shows that the PvuⅡ-HpaⅡ fragment (144bp) containing the promoter and signal peptide sequence from pYK190 plasmid is sufficient for expression and secretion of foreign genes in E. coli. The deletion of the dyad sequence (26bp) from 5' end of the promoter region doesn't affect expression level of foreign target genes. The experiment also provids the useful information for construction of efficient expression and secretion vector using the PvuⅡ-HpaⅡ fragment and studying regulation of gene expression.