This review summarizes the transcriptional regulations in the eucaryotic genes transcriped by three kinds of RNA polymerases. The regulatory strategies differ for higher eucaryotic cells with their huge DNA contents. First. much greater numbers of transcriptional factors are required. And second, these regulatory proteins simultaneously bind ot the nearby specific sites on DNA with proper orders. This demonstrated that the control of transcription in eucaryotic cells involves the interaction of protein factors with specific DNA sequence elements and the interactions between protein factors.