On analyzing the distribution and composition of element in single cell with scanning proton microprobe (SPM) and synchrotron radiation X-Ray fluoroscence microprobe (SR-XMF) it shows that zinc is a constituent of cell plasma. The levels of cellular zinc and iron were detectecd by above nuclear technique and the contents of MDA, SH group were estimated respectively by biochemical method for both normal and injured cell induced an peroxidation damage by hydroxyl-free radical. It was found that MDA content increased, SH group decreased. as well as Fe / Zn ratio raised during lipoperoxidation. By supplementation of zinc to culture medium. the inhibitory effect of zinc on lipoperoxidation was obvious from following experiment results that MDA content decreased. SH group content increased as well as Fe / Zn ratio reduced. The results suggest that zinc plays a role in maintaining the integrity of the cell and protects SH group of membrane protein thus preventing catalytic peroxidation reaction of iron.