Biological high resolution electron microscopy,a method developed recently, is comparable to X-ray crystallography for determination of high resolution structure of biological macro-molecules. It overcomes some difficulties confronted by X-ray crystallography and can apply directly to the non-crystal biological macro-molecules or to those proteins which can only form two-dimentional crystals. This method contains mainly experimental recording of real structure information and image analysis of the electron micrographs. Several problems which will be encountered in the application of these techniques,e.g.natural structure preservation, radiation damage, poor contrast, and low signal-noise ratio are discussed.