Gangliosides are one of the glycosyl-ceramides, which are particularly abandant in the central nervous system of vertebrates. The content and components of pig brain Gls were detected. There were 0.0894% (W/W) Gls presented in the pig brain. It was about 0. 39% (W/W) of total lipid. The main components were GM1. GD3, GD1a, GD1b and GT1b. GM1 and GD1a of the pig brain were higher than that of human brain.