A Rapid and Reliable Method for Direct Sequencing of PCR Products

Home > Archive>Volume 21, Issue 5, 1994 >458-459

A Rapid and Reliable Method for Direct Sequencing of PCR Products
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A simple, rapid and reliable sequencing method for double stranded PCR products is described. This method presented utilizing the unique property of T7 DNA polymerase which remains active at low temperature to allow the sequencing reaction performed at low temperature. Excellent sequencing results have been obtained by this method for various PCR products .

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Wang Liang, Zhang Jinsan, Zhu Dan, Yin Luo, Wang Xiuqin, Wu Min. A Rapid and Reliable Method for Direct Sequencing of PCR Products[J]. Progress in Biochemistry and Biophysics,1994,21(5):458-459

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Received:June 29,1994
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