Being as an efficient expression vector,baculovirus is now being used to express a wide variety of genes from viruses,fungi,plants and animals. However,some short-comings,such as low efficacy of homologous recombination and difficulty as well as long time consumed in puirfication of recombinant baculoviruses,accompany the traditional method of constructing a recombinant baculovirus. To overcome the shortcomings,some new and efficient techniques,including linearizing viral DNA to increase the ratio of recombinant baculoviruses recombinating foreign genes in vitro. completing homologous recombination and selection of recombinant viruses in yeast and in E. coli, and making recombinant viruses to produce polyhedra, have been developed in recent years to construct the recombinant baculoviruses rapidly and conveniently. These improved methods are. therefore. to be described.