Kumming mice were chosen as experimental anminals.Under the conditions of actue and repeatitive hypoxia and normal,the indivival relative percentage of ganglioside GM1,GD1a,GD1b and GT in the brain were measured by the CS-9000 thin-layer-scanning photometer respectively. Comparing with the group A of blank control without exposure to hypoxia , group B of experimental control exposured to hypoxia only once, and group D of another experimental control fed for two days after acute and repeatitive exposure to hypoxia for 4 times，the content of sialic acid significantly decreased and that of GT markedly increased in the experimental animals group C acutely and repeatedly exposured to hypoxia for 4 times;while the contents of ganglioside were also different between group A and D significantly. The results indicate that ganglioside in the brain may be involved in the development of tolerance to hypoxia in animals exposured to acute and repeatitive hypoxia and the proceeding was not a fast respone which may be restored.