Protein specific-cleavage reagents refer to those cHemical tools which can cut off peptide-bonds specifically.These regents can be divided into two classes. The first is called oxidative cleavage reagents. Oxidative cleavage has the property of stereo-specificity. Only the peptide bonds which close to cleavage system in tertiary structure are cleaved. The second is called hydrolytic cleavage reagents.These complexes can catalyze the hydrolysis of specific peptide bonds directed by protein sequence specificity.Protein specific-cleavage regents are useful for protein sequence analysis，for studies on the relationship between structure and function of protein and for synthesis of new chemical therapy drugs. The development of these reagents will improve the studies on protein chemistry and other relative subjects.