General reverse transcription polymerase chain reaction(RT-PCR)is a complicated process with low efficiency.And high quality mRNA is required and the mRNA is easily destroyed by RNase.The hybridoma cells were fixed with 10% formaldehyde solution in normal saline and permeabilised by 0.5% Nonidet P-40 in water. After the mRNA was reversely transcribed to cDNA and the cDNA was amplified by polymerase chain reaction(PCR),a specific heavy chain variable region gene(VH)of immunoglobulin with length of about 350 bp,being consistent with the product amplified by normal RT-PCR，was obtained. This technique could be applied to prepare specific structure gene fragmenu，to link and amplify chimeric protein genes and to construct human antibody lihraries.