Introduce a Rapid Method to Screening and Analysis of a cDNA Library

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Introduce a Rapid Method to Screening and Analysis of a cDNA Library
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In situ hybridization is a necessary and hard work for screening cDNA library.Heated by microwave oven,bacteriophage lysis,DNA denaturation and fixation of DNA to filter occur in a single step;On the other hand,it is a really rapid procedure for analysis of the cDNA inserts of positive clones via polymerase chain reaction (PCR) amplification.

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Zhou Zhaolan, Zhu Zhen, Liu Chunming, Xiao Guifang, Li Xianghui. Introduce a Rapid Method to Screening and Analysis of a cDNA Library[J]. Progress in Biochemistry and Biophysics,1995,22(6):565-566

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Received:December 17,1994
Revised:February 21,1995
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