Foreign porcine growth hormone genes were introduced into Hubei white pigs. Inorder to avoid possible homology in metallothionein-Ia gene promoter between sheep and porcine, two PCR primers were designed and located on the regions of sheep metallothionein Ia gene promoter and porcine growth hormone gene, respectively. This reflects the exact results by PCR detection. The results of DNA blotting indicated that 6 of 43 offsprings showed positive integration.
Liu Guisheng, Chen Qingxuan, Wang Jing, Wei Qingxin, Chen Yongfu. Identification of Integration of Foreign Gene in Transgenic Pigs[J]. Progress in Biochemistry and Biophysics,1996,23(3):279-280
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