The coding sequence of glial cell linederived neurotrophic factor (GDNF) was amplified from human genomic DNA by PCR method.Recombinant GDNF was expressed in E. coli and purified.
Chen Yan, Zhang Ying, Li Jinzhao, Deng Wei, Xia Lingchao, Qiu Rong. Cloning of Glial Cell Line-Derived Neurotrophic Factor Gene and Purification of Expressed Product[J]. Progress in Biochemistry and Biophysics,1996,23(6):556-557
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