Antisense oligonucleotides can bind to a specific viral RNA, and hence inhibit the reproduction of virus selectively. Six tetradecyl modified antisense phosphothioate deoxyoligonucleotides were synthesized, antiviral assay showed that RDS-53, RDS-32 and RDS-33 targeting to the translation initiation site, 3′-repeated and 3′-terminal sequences of D2-04 RNA had fairly strong inhibitory activity.