The rat liver tRNAIle gene has been synthesized before. Now the synthetic tRNAIle gene was transcripted in vitro with T7 RNA polymerase and the optimum reaction condition was investigated.
ZHANG Lu, ZHONG Xionglin, PENG Zhaohui, XU Qian. Transcription of Rat Liver tRNAIle Gene with T7 RNA Polymerase[J]. Progress in Biochemistry and Biophysics,1997,24(1):78-82
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