A solid phase and competitive enzyme immunoassay for the determination of microalbumin in urine using rabbit anti-human albumin antibody, HRP-HSA and Nunclon microtiter strips was developed. Two chromogens: o-phenylenediamine (OPD) and 3,3,5,5-tetremethyl benzidine (TMB), sodium carbonate coated wells and glutaraldehyde coated wells was compared. The influences on the determination of the concentration of substrated, antigen and antibody was discussed. The optimal conditions for the assay was established.