Purified recombinant Fusarium oxysporum cytochrome P-450nor (rF·P-450nor) was used for spectroscopic and kinetic studies. K_m and V_max were determined. K_m(NO)and K_m(NADH) were 0.128 mmol/L and 0.208 mmol/L respectively. V_max for N₂O was 11 363 min^-1. The spectroscopic study indicated that: rF·P-450nor has the typical characteristics of a heme protein, and has the maximum absorbance at 413 nm. After added Na₂S₂O₄，the maximum absorption peak was moved to 405 nm. After added CO and Na₂S₂O₄, the absorption peak was at 450 nm. When combined with NO, the absorption peak was moved to 430 nm. These spectrum characteristics were the same as those of the native F·P-450nor.