in situ Polymerase Chain Reaction of Human′s Single-copy and Repeated DNA Sequences

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in situ Polymerase Chain Reaction of Human′s Single-copy and Repeated DNA Sequences
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Single-copy and specific repeated DNA sequences of human Y chromosome were amplified and detected by in situ PCR in cultured human small intestine cancer metastastic ascites cell line cells. The results show that in situ PCR is much more sensitive than in situ hybridization.

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MA Qi, ZHANG Xiyuan, LIU Ting, YANG Jianqi, XU Yaoxian.in situ Polymerase Chain Reaction of Human′s Single-copy and Repeated DNA Sequences[J]. Progress in Biochemistry and Biophysics,1997,24(4):348-352

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Received:June 28,1996
Revised:October 21,1996
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