Sarcoplasmic reticulum Ca²⁺-ATPase proteoliposomes were reconstituted with ganglioside GM3 and soybean phospholipid by addition of detergent, sonication and chromatography. It was observed that GM3 may enhance the activity of Ca²⁺-ATPase in proteoliposomes and increase the diameter of uniform protein particles in sealed-well proteoliposomes vesicles which was evidenced by the negative-staining microscopy and freeze-fracture replica.