The proteins expressed by the glutathione S-transferase(GST) supergene family which catalyze the conjugation of endogenous or exogenous electrophiles with glutathione(GSH) are a family of detoxicating proteins,the gene expression of which is regulated by many sorts of compounds through different mechanisms. A brief review for the gene structure and regulation mechanisms associated with the expression of GSTs,and the action of oxidative stress in the regulation of GST gene expression was made.