In order to clone cDNAs coding for proteins specially binding to NF-IL6's 3′UTR, a phagemid expression library was constructed from cDNAs of revertant RR cells. The termination codons of those cloned cDNAs were largely removed by restricted exoⅢ enzyme digestion, in order to facilitate the expression of cDNA as fusion proteins with the phage gene Ⅲ. Examinations of this library showed that the library includes various lengths of cDNA inserts and it can express exogenous cDNA.
HE Yuanzheng, NI Xiaodong, LIU Dinggan, QING Guoliang, LI Zaiping. Cloning cDNA of RNA-binding Proteins by Using Phage Display Method: Construction of the Phage Expression Library[J]. Progress in Biochemistry and Biophysics,1997,24(5):467-470
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