Reverse dot blot (RDB) technique was used. Seven sequence-specific oligonucleotide (SSO) probes directed against 7 types of HPV (HPV6B, 11, 16, 18, 31, 33 and 35) respectively are synthesized and fixed sequentially on a stripe of nylon membrane to form 7 spots. After hybridization with the PCR product of the sample DNA sequence, any one of the 7 types of HPV DNA were distinguished on only one stripe of nylon membrane. 38 cases of cervical carcinoma samples were detected by this PCR-RDB technique. The results show: 29 cases (76.3%) of HPV16 positive; 8 cases (21.1%) of HPV18 positive; 2 cases (7.2%)of double infection; and 3 cases (8.0%) of HPV negative.The method is rapid and simple, with high specificity, no false positive results in general, and as the PCR technique is very sensitive, the method is not liable to show false negative results.