Recently, attention has been focused on the association between neuronal apoptosis and neurodegenerative diseases during the deepgoing study of apoptotic cells. Many in vitro models of neuronal apoptosis have been developed, such as induced by nutrition deprivation, free radicals, glutamic acid, Ca²⁺ ,β-amyloid peptide etc. The detective technology of neuronal apoptosis is composed of testing the changes in enzymes and proteins which reflected neuronal damage, simultaneous morphological observation, and finally DNA electrophoresis etc, which could confirm the apoptosis.