There are a lot of difficulties using PCR method to identify low copys of exogenous gene in transgenic animal. A method of agarose gel direct hybirdization was established. A fast and easy method to identify low copy transgenes in transgenic animal is provided.
LU Yi-fan, TIAN Chai, DENG Ji-xian. A Rapid Method of Identifying Low Copy Number Transgene Using Agarose Gel Hybirdization[J]. Progress in Biochemistry and Biophysics,1998,25(3):269-271
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