The first intron sequence of human α1(Ⅰ) collagen (COLⅠA1) gene has been shown to have differential activities of transcription regulation in a variety of tissue cells. To determine the activity of the first intron of COLⅠA1 gene and then utilize the regulatory elements to perform ectopic expression of foreign genes in human fibroblasts. Two novel recombinants pSCEP-CAT and pSCIP-CAT were constructed in which the intronic +544～+855 and +820～+1 093 segment of COLⅠA1 gene were cloned respectively in the upstream position of the promoter sequence of this gene followed by chloramphenical acetyltransferases (CAT) gene. Human fetal tendon fibroblasts and Tca 8113 tongue cancer cells in culture were transfected with the recombinants by using LipofectAMINE^TM, and then tested the expression level of CAT gene with DIG-labeled anti-CAT ELISA. The results indicate that the first intronic +544～+855 segment of COLⅠA1 gene has an enhancing effect on the expression of CAT gene driven by the promoter in the tranfected human fetal tendon fibroblasts and Tca 8113 tongue cancer cells. Interestingly, the first intronic +820～+1 093 segment has strongly stimulating effect on the expression of the marker gene in the tongue cancer cells, but inhibitory effect in the fibroblasts.