A modified method for seperating neutrophils from whole blood was reported. Neutrophils was obtained by dextran sedimentation, centrifuged with lymphocytes separating solution, special seperting medium washing and erythrocyte depletion. The purity and viability were proved to be both about 95% by Wright's stain and Typan blue respectively. Both the chemiluminescence method and cytochrom c reduction method proved the high activity and the integrity of the membrane receptor of the neutrophils. Compared with other method, this method is more simple and easier to handle, which suggests that it is a efficient and economic method for seperating neutrophils.