Auxilin is a molecular chaperon which induces binding of Hsp70c to clathrin, playing an important role in uncoating of coated vesicle isolated from brain. Through integrated analysis of public database such as dbEST, dbSTS, partial cDNA sequence of human auxilin was identified and mapped to 1p31, between marker D1S515 and D1S198. Totally 26 ESTs were found to be part of human auxilin and used to construct five contigs, which make totally 2.3 kb sequence and contains 501 bp coding sequence. All the sequences obtained and the corresponding putative translation showed high homologous to bovine auxilin. At the same time, EST data indicate that human auxilin expressed in several tissues at fetal stage, also in brain and melanocyte at adult stage.