By utilizing imported chemiluminescent reagent, self-prepared rabbit anti-human type Ⅳ collagen IgG and a horseradish peroxidase-labeled anti-human type Ⅳ collagen monoclonal antibody, a method of sandwich chemiluminescent enzymimmunoassay(CLEIA) was established then evaluated. The results showed the CLEIA has a lower detecting limit of 30 μg/L，the average recovery was 94.5％, CVs of intra-assay and inter-assay were 6.0％ and 11.6％ respectively, the CLEIA method has a good correlation with Japan kits (ELISA, r=0.9686). the serum reference level by testing 60 cases health is 160 μg/L. It was found that the CLEIA is a sensitive, acurate, stable and specific method，it may be a qualified candidate for serum type Ⅳ collagen detemination of non-radioactive method.