The change of quality and quantity of wound fluid during different stages could reflect the course of wound healing in a certain degree. The effect of WF taken from different post wounding days on the proliferation of mouse wound fibrolast(mWFb) was studied, and the regulatory role of wound microenvironment on the growth of mWFb was also investigated. It was performed in two kinds of culturing medium: 1640-1%FCS-10%WF. It was found that WF obtained from postwounding days 1,3 and 7 could effectively stimulate the proliferation of mWFb, while, WF obtained from postwounding days 9,11 and 15 with 10%FCS presented inhibitory effects on the proliferation of mWFb. However, the same WF with 1%FCS may induce death of mWFb. The results showed that wound microenvironment could stimulate the proliferation of mWFb within the first week after wounding, and that cell growth may be arrested by WF of the wound during the second week. These might be some growth inhibitory factors in the wound microenvironment of later stage during wound healing.