A TRAP Assay on Human Colonic Tumor Telomerase Activity

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A TRAP Assay on Human Colonic Tumor Telomerase Activity
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Abstract:

A TRAP assay on human colonic tumor telomerase activity is reported. The reliable result is obtained when 1.8 mmol/L Mg²⁺ and 55℃ annealing temperature are adopted. The other two factors affecting reliability are quantity of the extract and contamination of the positive DNA fragments in the reaction.

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GU Shao-Hua, JIANG Guang-Ping, CAO Gen-Tao, SHENG Ji-Qun, LIU Jian-Ping, DAI Jian-Liang, LIN Qing, LIN Yuan, LI Shi-Zhong, LIU Wei-Ping, XIE Yi. A TRAP Assay on Human Colonic Tumor Telomerase Activity[J]. Progress in Biochemistry and Biophysics,1999,26(1):72-75

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Received:September 22,1997
Revised:January 16,1998
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