Epatein-Barr Virus(EBV) is isolated from B95-8 cell line, the primary B lymphocytes from human blood and tonsil tissue are transfected with EBV in order to gain immortalized lymphoblastoid cell lines(LCLs) and tonsil lymphoblastoid cell lines(TLCL). The LCLs and TLCL contain wt p53 gene, and do not express p53 proteins before treatment with cisplatin. Cell viability are decreased, p53 proteins are accumulated,and DNA ladders appear in LCLs and TLCL with time extension after treating with 10 mg/L. Lymphoma cell lines(BJAB, Raji, Ramos, Molt4) carring mutant p53 gene show accumulation of p53 protein before treatment with cisplatin. In paralleled experiment, the lymphoma cell lines appear relatively resistant to the doses of cisplatin. The results suggest that cisplatin cause DNA damage of LCLs and TLCL, activate wt p53 expression, and cells with DNA damage enter the pathway of apoptosis induced by wt p53.