Human immunoglobulin heavy chain and light chain genes were separately amplied by PCR with cDNA as templates from human peripheral lymphocytes，using four 5′-primers and four 3′-primers corresponding to heavy chain, eight 5′-primers and two 3′-primers corresponding to light chain. At different annealing temperature, using the primers described above, all the light chain PCR products and 80% heavy chain PCR products were yielded. When signal sequences of immunoglobulin were used as 5′ primers, all remain heavy chain products were obtained by this half-nested PCR. This results indicate that more immunoglobulin variable region genes can be obtained by PCR through changed of procedures and reaction conditions, which can increased the diversity of antibody library.