Intact goat small antral follicles were dissected from ovaries by a micro-surgical method. The follicular DNA content was estimated by a fluorometric method using the fluorochrome, 4,6-diamidino-2-phenylindol-2HCl(DAPI).Significant positive correlations were found between the follicular diameters and follicular DNA contents in follicles smaller than 3 mm (r=0.9824＞0.7800=r_0.001,n=12). It suggested that this method of DNA assay would have great application value in study on follicular gowth and development.