A new method for PCR products cloning——T-A cloning technique is introduced. pBluescript SK(+) plasmid was extracted by modified alkali lysis method. A blunt ends was generated using restriction enzyme EcoRⅤ, then T-A cloning vector was prepared in the presence of Taq DNA polymerase and dTTP. β-actin cDNA fragment was cloned into the above T-A cloning vector. After the recombinant plasmids were digested by EcoRⅠ and HindⅢ, an expected size of β-actin cDNA fragment was obtained.