Total 40 random primers of OPN and OPM groups made in Operon Co. were applied to the RAPD analysis of H.molitrix and A.nobilis. Besides establishment of species-specific RAPD electrophoretogram, some kinds of primers which can produce molecular genetic markers of RAPD with individuality- or population-specificity of these fish were found. A modified method to extracting genomic DNA from peripheral blood cells of fish, and the application prospects of RAPD technique in genetic diversity and variation of fish, and in fishery management and the evaluation of fish resources were discussed.