A method for purifying HA from ox eyes was studied.Using 0.125 mol/L Na₂SO₄ other than 0.1 mol/L NaCl to dissolve the semifinished product, Two HA forms in Na₂SO₄'s solution were found. One in staple could be absorbed by CBP, and the other in powder could be returned by fractional dilution afterwards. In 0.1 mol/L NaCl solution, HA could only be recovered by CBP absorption. It displayed staple. The recoverable rate of the method was 1.7 times than others.The physical and chemical characters of the product were analysed. It was proved that higher purity recovery and lower cost than other methods were gained.