To investigate the gene expression of growth factor Wnt-5A in different phases during cell cycle at transcriptional level.To synchronize the renal cell carcinoma GRC-1 cell line was by double thymidine blocks and high-pressure N2O gas methods. Using Semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction) to amplify the cDNAs of Wnt-5A in different phases. Result showed that the mRNA expression of growth factor Wnt-5A was detected in RCC GRC-1cell line. In S phase, the highest level of Wnt-5A was observed, medial and lowest was in G1 and M phase, respectively. The differences between S and M stages were significant (P＜0.05).Therefore growth factor Wnt-5A has the potential effect on tumorigenesis, It contributes to all phases during cell cycle, but gives more influence to S phase during cell cycle.