Ultra-weak chemiluminescence analytical technology were used to research of oncology. The luminescence intensity of serum and urine of bone tumor patients and normal persons were measured by BPCL ultra-weak luminescence analyzer. The results showed that the luminescence intensities of serum and urine of bone tumor patients were higher than those of the normal subjects (P＜0.05). The urine luminescence intensity of bone tumor patients significantly decreased after operation (P＜0.05). The luminescence from blood and different organs of nude mice were measured. The results showed that the luminescence intensities from different organs increased greatly after inoculation.