To establish a flow cytometric method (FCM) for simultaneous evaluation of asialoglycoprotein receptor (ASGPR) on the surface of normal rat hepatocytes, injured rat hepatocytes and hepatoma cells (BEL-7402). FCM for ASGPR was established using normal hepatocytes (L-02) and FITC-conjugated galactosyl-neoglycoalbumin (FITC-NGA), the specific ligand for ASGPR. The mean intensity of fluorescence (MIF) of the three different hepatocytes were determined and calculated after simultaneously incubated with FITC-NGA at the same concentration. The concentration of FITC-NGA for saturating ASGPR on the surface of L-02 was 0.4 mg/L, at which the MIF of the three different hepatocytes were 228.7，5.81 and 1.13 respectively. The saturated combination can be completely inhibited by 50-fold NGA or 10mmol/L EDTA. FCM can display soundly the characteristic of the receptor-ligand combination between ASGPR and FITC-NGA. Compared with normal rat hepatocytes, there is no ASGPR on the surface of BEL-7402, and the quantities of ASGPR on the surface of injured rat hepatocytes decreases significantly.